Solubilization and reconstitution of the melibiose carrier from a plasmid-carrying strain of Escherichia coli.
نویسندگان
چکیده
A strain of EScherichia coli was constructed containing a plasmid from the Clarke-Carbon collection that showed high levels of melibiose transport activity. Membranes from the plasmid-containing strain were extracted with octyl-beta-D-glucopyranoside and melibiose transport was reconstituted in liposomes. The proteoliposomes exhibited counterflow activity, as well as membrane potential and sodium gradient-driven melibiose accumulation.
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 257 9 شماره
صفحات -
تاریخ انتشار 1982